BIO 250 Mcorbiology (Micro)
Spring 2015
Current Reading Assignments and Problems (CRAaP)
Note: this page is a dynamic study guide; new entries may appear with
each new slide set (SS) added to the exam box.
Important NOTEs: (a) the first slide of each slide set also displays all the current Reading Assignments;
(b) slide set names are the same as in your I:/drive folders
| Slide Set (SS) | Reading (RA) | Articles & HOs | EOC problems | Labs |
|---|---|---|---|---|
| (SS1)Bio250Ch01.pptx | ||||
(previous year - older text edition)
Slide-Sets (SS)
|
Comments on slide sets |
| (SS1): Bio250Ch01.ppt |
Microorganisms as cells and the Properties of Life; spontaneous generation and Pasteur's experiments; germ theory of disease, Koch's postulates Review of the Scientific Method (hypothetico-deductive method): from explanation to theory, induction/deduction; reaching generalizations, testing hypotheses, making theories |
| (SS2):Bio250Ch02.ppt | Cell structure & Evolutionary history with microbial diversity; Domains classifications scheme for the Tree of Life; ; |
| (SS3):Bio250Ch04(I).ppt | Microscopy & Cell morphology; the importance of being small |
| (SS4):Bio250Ch04(II).ppt | The structure of cell walls and its implications for classification, morphology, physiology, and the gram stain reaction; the structure of cell membranes and implications for function |
| Readings - Text | Readings - Articles, Handouts |
| ch.1(p.2-5; p.9-15) | The Undiscovered Planet and Questions; Archaea article about Carl Woese and Ralph WolfCh.1,3, and 4 Working Glossary Terms (assigned ones on slides) |
| ch.2(p.22-26) cell size & morphology, some external structures; (p.26-27) the Tree of Life; (p.28-35) microbial diversity | |
| ch.4partI(p.56-60) Light microscopy; (p.63-66) cell morphology and the significance of being small | |
| ch.4partII(p.66-71) cell membrane structure & function; (p.74-82) cell wall structure; osmotic pressure; outer membranes | |
| EOC (end-of-chaper) Problems | Labs |
| Review Questions, ch.1 (RQ1): 1-3, 6-10; Applicaton Questions, ch.1 (AQ1): 1, 2 | (and see also your Lab Schedule page on this WSS) |
| RQ ch.2 (1-4); 6-10, 12; AQ: 2-4 | |
| RQ ch.4 partI (1, 2, phase only), 4; AQ: 1; ch.4 partII (5,6,7,9); AQ: 2 | |
Slide-Sets (SS) |
Comments, Articles, HOs |
| (SS5):Ch05PrtA | First of 3 parts (A, B, C) for chapter 5; fundamental energy concepts including basic thermo- dynamics, biochemical pathways, metabolism; properties of energy-rich molecules, essential enzymology |
| (SS6):Bio250Ch05PrtB | Energy carriers; redox reactions; standard reduction potentials; coupled reactions; connecting thermodynamics to electrochemistry |
| (SS7):Bio250Ch05PrtCa | Major catabolic pathways: glycolysis and CAC; what goes into them, what comes out of them, and keeping track of the energetics by thermocyanmics and elecrtrochemistry; electron transport, the proton motive force and oxidative phosphorylation; metabolic diversity in microbes |
| (SS8):BIO250Ch06PartA | The mathmatcis of bacterial growth; measuring bacterial growth directly and indirectly |
| (SS9):BIO250ch06PartB | (not covered - except for slides 7-10 which you must review on your own time - not in class) |
| (SS10):BIO250Ch07PartA - up to slide #9 | The Central Dogmo of Molecular Biology; cells as coding machines |
| Reading Assingments (RAs)- Text | |
| RA for (SS5): ch.5 (p.108-112; p.130, fig. 5-24) | |
| RA for (SS6): ch.5 (p. 112-117; and see Appendix 1, p. A-4) | see the more expanded Standard Reduction Potentials "tower" |
| RA for (SS7): ch.5 (p. 117-130) | |
| RA for (SS8): ch.6 (p. 136-137; 140-148) | |
| RA for (SS9): ch.6 (p. 161-164) | Rsection in chapter 6 which pertains to slides 7-10 in (SS9) |
| RA for (SS10): ch. 7: (p. 166-169) | |
| EOC (end-of-chaper) Problems | Labs |
| EOC for ch. 5: RQ 4-19 | (and see also your Lab Schedule page on this WSS); exercise #21, then Enterotube and any enterotube assay presented in exercises #23 and #24: |
| EOC for ch. 6: RQ 4-8, 16; AQ 1, 2 | |
| EOC for ch. 7: RQ 1 | |
| Slide sets (SS), Lab work | Comments, articles, HOs |
| (SS10):BIO250Ch07PartA - start with slide #10 | DNA, gene, crhromosome, genome (genetic element) structure; DNA replication molecular mechanisms |
| (SS11):BIO250Ch07PartB - PCR & DNA Sequencing | PCR and DNA sequencing |
| (SS12):BIO250Ch07PartC - Gene Anatomy & Structure; transcription & the Genetic Code | RNA structure and function; transcription molecular mechanism; genetic code |
| (SS13):BIO250Ch08 - Gene/Metabolic Regulation | Gene regulaton by reprssors, activators, sigma factors, and RNA; negative and positive control mechanisms; operons, regulons, modulons, stimulons, and global control circuitry; HSP response to stress; anti-sense RNA and riboswitches |
| (SS14):BIO250Ch10PartA - Bacterial Genetics: Mutations | Mutations, mutants, and mutagenesis; nature of mutations, mutant isolation (replica plating), mutagenesis mechanisms; the Ames Test |
| (SS15):BIO250Ch10PartB - Bacterial Genetics - Gene exchange and transfer mechanisms | Transformation, transduction, conjugation; cell competency; plasmids, F-plasmids, R-factors and antiobiotic resistance; transposons |
| Reading Assingments (RAs) - Text | |
| RA for (SS10): ch.7 (p.169-180) | DNA structure, inverted repeats, Tm's; DNA replication mechanisms, template-primer, ori's and replication forks, major enzymes in DNA replication |
| RA for (SS11): ch.7 (p.184-188) | PCR molecular mechanism; reaction steps and cycles; DNA sequencing molecular mechanism; automated DNA sequencing |
| RA for (SS12): ch.7 (p.188-193; 193-198) | transcription initiation and termination; RNA polymerase structure and function; ribosome structure - genetic code, tRNA structure and function |
| RA for (SS13): ch.8 (p.206-207; 212-222; 228 & fig. 8.28) | |
| RA for (SS14): ch.10 (p. 257-264; 266-267) | handouts on replica plating and the Ames test |
| RA for (SS15): ch.10 (p. 268-274; 274-277; 279-281; 284-287) | handouts on transposons; general biology of insertion sequences and transposons (no detailed molecular mechanisms); mutagenesis with transposons |
| RA for (SS16); ch.20 (p.681-687); ch.21 (716-721); ch. 22 (p. 729-736; 767-768) | antibiotics; virulence factors and toxins; overview of the immune system; MHC genes; antibody structure and function |
| EOC (end-of-chaper) Problems | |
| EOC for ch.7: RQ 3-5; 8,9; 11-17; AQ 1-4 | |
| EOC for ch.8: RQ 5, 6, 8, 13 | |
| EOC for ch.10: RQ 1-8; 10, 12-14; 17, 22 |