Some commonly used Sequencing Primers

(a) Certify that your chosen sequencing primer is complementary to a site in your plasmid vector

(b) Compare a forward against another forward, or a reverse against another reverse, and note the similarities and differences (usually extra nucleotide bases at the 5’ or 3’ ends)

(c) What’s most important is to certify that your selected primer is exactly complementary to a target priming site in your plasmid vector, AND WILL PRIME SYNTHESIS TOWARDS YOUR INSERT!

(d) full maps with priming target sites for all cloning vectors are available from the vendor’s web-site, or just try a Google with your particular plasmid vector’s name or description

Universal Reverse 20mer 5' CACAGGAAACAGCTATGACC 3'

Universal Forward 20mer 5' GTTGTAAAACGACGGCCAGT 3'

M13 Forward Sequencing Primer (-21)
5’ GTA AAA CGA CGG CCA GT 3’

M13 Reverse Sequencing Primer (-28)
5’ AGG AAA CAG CTA TGA CCA T 3’

M13 Forward Primer (-40)
5’ GTT TTC CCA GTC ACG ACG 3’

M13 Reverse Primer (-29)
5’ CAG GAA ACA GCT ATG ACC 3’

T7 Promoter Primer
5’ TAA TAC GAC TCA CTA TAG GG 3’

SP6 Promoter Primer
5’ ATT TAG GTG ACA CTA TAG 3’